Investigation of non-covalent complexes of HIV-1 promoter DNA and polyamides containing N-methylpyrrole by electrospray ionization mass spectrometry
Article first published online: 4 MAY 2006
Copyright © 2006 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 20, Issue 11, pages 1736–1740, 15 June 2006
How to Cite
Li, H. and Yuan, G. (2006), Investigation of non-covalent complexes of HIV-1 promoter DNA and polyamides containing N-methylpyrrole by electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom., 20: 1736–1740. doi: 10.1002/rcm.2508
- Issue published online: 4 MAY 2006
- Article first published online: 4 MAY 2006
- Manuscript Accepted: 25 MAR 2006
- Manuscript Revised: 22 MAR 2006
- Manuscript Received: 1 FEB 2006
- National Natural Science Foundation of China. Grant Number: 20272005
- Research Fund for the Doctoral Program of Higher Education
Eight novel polyamides containing N-methylpyrrole were designed to target the sequence (5′-CTGCATATAAGCAG-3′/5′-CTGCTTATATGCAG-3′) of the TATA box element of the HIV-1 promoter DNA. The non-covalent complexes of the promoter DNA and the polyamides were investigated by electrospray ionization (ESI) mass spectrometry, which provided strong evidence for the binding of the novel polyamides to the sequence of the TATA box element. It also revealed that polyamide 2 (PyPyPyPyβDp), a potent binder of HIV-1 promoter DNA and a lead molecule for the design of new anti-HIV-1 drugs, had the highest binding affinity with the TATA box element DNA among these polyamides by examining the stoichiometry and the selectivity. Copyright © 2006 John Wiley & Sons, Ltd.