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Mass spectrometric identification and characterization of a new long-term metabolite of metandienone in human urine

Authors

  • Wilhelm Schänzer,

    1. Center for Preventive Doping Research–Institute of Biochemistry, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany
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  • Hans Geyer,

    1. Center for Preventive Doping Research–Institute of Biochemistry, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany
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  • Gregor Fußhöller,

    1. Center for Preventive Doping Research–Institute of Biochemistry, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany
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  • Natalia Halatcheva,

    1. Doping Control Laboratory, Ministry of Youth and Sport, Directory ‘Sport and Health’, Nicola Gabrovsky 1, 1172 Sofia, Bulgaria
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  • Maxie Kohler,

    1. Center for Preventive Doping Research–Institute of Biochemistry, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany
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  • Maria-Kristina Parr,

    1. Center for Preventive Doping Research–Institute of Biochemistry, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany
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  • Sven Guddat,

    1. Center for Preventive Doping Research–Institute of Biochemistry, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany
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  • Andreas Thomas,

    1. Center for Preventive Doping Research–Institute of Biochemistry, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany
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  • Mario Thevis

    Corresponding author
    1. Center for Preventive Doping Research–Institute of Biochemistry, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany
    • Center for Preventive Doping Research–Institute of Biochemistry, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany.
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Abstract

Anabolic-androgenic steroids are some of the most frequently detected drugs in amateur and professional sports. Doping control laboratories have developed numerous assays enabling the determination of administered drugs and/or their metabolic products that allow retrospectives with respect to pharmacokinetics and excretion profiles of steroids and their metabolites. A new metabolite generated from metandienone has been identified as 18-nor-17β-hydroxymethyl,17α-methyl-androst-1,4,13-trien-3-one in excretion study urine samples providing a valuable tool for the long-term detection of metandienone abuse by athletes in sports drug testing. The metabolite was characterized using gas chromatography/(tandem) mass spectrometry, liquid chromatography/tandem mass spectrometry and liquid chromatography/high-resolution/high-accuracy (tandem) mass spectrometry by characteristic fragmentation patterns representing the intact 3-keto-1,4-diene structure in combination with typical product ions substantiating the proposed C/D-ring structure of the steroid metabolite. In addition, structure confirmation was obtained by the analysis of excretion study urine specimens obtained after administration of 17-CD3-labeled metandienone providing the deuterated analogue to the newly identified metabolite. 18-Nor-17β-hydroxymethyl,17α-methyl-androst-1,4,13-trien-3-one was determined in metandienone administration study urine specimens up to 19 days after application of a single dose of 5 mg, hence providing an extended detection period compared with commonly employed strategies. Copyright © 2006 John Wiley & Sons, Ltd.

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