Quantification of tizanidine in human plasma by liquid chromatography coupled to tandem mass spectrometry
Article first published online: 29 JUN 2006
Copyright © 2006 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 20, Issue 15, pages 2286–2292, 15 August 2006
How to Cite
Nirogi, R. V. S., Kandikere, V. N., Shukla, M., Mudigonda, K. and Maurya, S. (2006), Quantification of tizanidine in human plasma by liquid chromatography coupled to tandem mass spectrometry. Rapid Commun. Mass Spectrom., 20: 2286–2292. doi: 10.1002/rcm.2592
- Issue published online: 29 JUN 2006
- Article first published online: 29 JUN 2006
- Manuscript Revised: 31 MAY 2006
- Manuscript Accepted: 31 MAY 2006
- Manuscript Received: 17 APR 2006
A simple, sensitive and rapid high-performance liquid chromatography/positive ion electrospray tandem mass spectrometry (MS/MS) method was developed and validated for the assay of tizanidine in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reversed-phase column and analyzed by MS/MS in the selected reaction monitoring mode. The assay exhibited a linear dynamic range of 50–5000 pg/mL for tizanidine in human plasma. The lower limit of quantification was 50 pg/mL with a relative standard deviation of less than 13%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.5 min for each sample made it possible to analyze more than 300 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright © 2006 John Wiley & Sons, Ltd.