A fast liquid chromatographic/mass spectrometric screening method for the simultaneous detection of synthetic glucocorticoids, some stimulants, anti-oestrogen drugs and synthetic anabolic steroids

Authors

  • Monica Mazzarino,

    1. Laboratorio Antidoping, Federazione Medico Sportiva Italiana, Largo Giulio Onesti 1, 00197 Rome, Italy
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  • Francesco Botrè

    Corresponding author
    1. Laboratorio Antidoping, Federazione Medico Sportiva Italiana, Largo Giulio Onesti 1, 00197 Rome, Italy
    2. Dipartimento CGMIA, Università ‘La Sapienza’, Via del Castro Laurenziano 9, 00161 Rome, Italy
    • Antidoping Laboratory of Rome, Largo Giulio Onesti 1, 00161 Rome RM, Italy.
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Abstract

A fast liquid chromatographic/mass spectrometric (LC/MS/MS) screening method for the detection, in urine, of synthetic glucocorticoids, stimulants (formoterol, modafinil and mesocarb), anti-oestrogens (finasteride, exemestane, anastrozole, letrozole and formestane) and synthetic anabolic steroids (stanozolol, gestrinone and tetrahydrogestrinone) is described. All these drugs (and/or their urinary metabolites) can be simultaneously extracted by a single liquid/liquid extraction step, at alkaline pH, after enzymatic hydrolysis with β-glucuronidase, and assayed in 7 min by LC/MS/MS using electrospray ionization in positive ion mode and multiple reaction monitoring as the acquisition mode. All compounds show good reproducibility of both the retention times (CV% <2%) and the relative abundances (CV% <10%). The limits of detection for the anti-oestrogens, glucocorticoids and steroids are in the range of 1–30 ng/mL, and for the stimulants are in the range of 100–200 ng/mL, thus satisfying the minimum required performance limits of the World Anti-Doping Agency. Copyright © 2006 John Wiley & Sons, Ltd.

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