A metabonomic analysis of plasma from Zucker rat strains using gas chromatography/mass spectrometry and pattern recognition
Article first published online: 17 OCT 2006
Copyright © 2006 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 20, Issue 22, pages 3295–3302, 30 November 2006
How to Cite
Major, H. J., Williams, R., Wilson, A. J. and Wilson, I. D. (2006), A metabonomic analysis of plasma from Zucker rat strains using gas chromatography/mass spectrometry and pattern recognition. Rapid Commun. Mass Spectrom., 20: 3295–3302. doi: 10.1002/rcm.2732
- Issue published online: 17 OCT 2006
- Article first published online: 17 OCT 2006
- Manuscript Revised: 5 SEP 2006
- Manuscript Accepted: 5 SEP 2006
- Manuscript Received: 17 JUL 2006
Plasma obtained from three strains of Zucker rats was analysed using capillary gas chromatography/mass spectrometry (GC/MS) to obtain global metabolite profiles as part of a series of metabonomic investigations of animal models of diabetes. Samples were obtained from 20-week-old male wild-type Zucker lean, (fa/fa) obese and lean/(fa) animals and were analysed following protein precipitation, using acetonitrile, and derivatisation. Subsequent data analysis using principal components analysis (PCA) and orthogonal projection to latent structures (OPLS) revealed differences between the plasma metabolite profiles of the three strains, with those of the Zucker lean and the lean/(fa) crosses being similar to each other whilst differing from the (fa/fa) obese strain. Copyright © 2006 John Wiley & Sons, Ltd.