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Liquid chromatographic/tandem mass spectrometric method for the quantitation of tranilast in human plasma



An analytical method for the determination of tranilast in human plasma using tramadol as the internal standard has been developed based on liquid chromatography/tandem mass spectrometry. Sample preparation involved protein precipitation with methanol. Separation by reversed-phase high-performance liquid chromatography using methanol/10 mM ammonium acetate (70: 30, v/v) as mobile phase was complete in a run time of 2.4 min. Detection on a Q TRAP™ system used multiple reaction monitoring. The method was linear in the range 0.06–20 µg/mL with intra- and inter-day precisions (as relative standard deviation) of 2.2–2.6% and 2.3–2.9%, respectively. Accuracy (as relative error) was <−2.5%. The method was applied in a pharmacokinetic study in healthy volunteers treated with a single 80 mg oral dose of tranilast. Copyright © 2006 John Wiley & Sons, Ltd.