Mass spectrometric detection of CP4 EPSPS in genetically modified soya and maize
Article first published online: 3 JAN 2007
Copyright © 2007 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 21, Issue 3, pages 319–328, 15 February 2007
How to Cite
Ocaña, M. F., Fraser, P. D., Patel, R. K. P., Halket, J. M. and Bramley, P. M. (2007), Mass spectrometric detection of CP4 EPSPS in genetically modified soya and maize. Rapid Commun. Mass Spectrom., 21: 319–328. doi: 10.1002/rcm.2819
- Issue published online: 3 JAN 2007
- Article first published online: 3 JAN 2007
- Manuscript Accepted: 2 NOV 2006
- Manuscript Revised: 30 OCT 2006
- Manuscript Received: 3 APR 2006
- GO1 Program of the UK Food Standards Agency
The potential of protein fractionation hyphenated to mass spectrometry (MS) to detect and characterize the transgenic protein present in Roundup Ready™ soya and maize has been investigated. Genetically modified (GM) soya and maize contain the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene from Agrobacterium tumefaciens CP4, which confers resistance to the herbicide glyphosate. The GM soya and maize proteomes were fractionated by gel filtration, anion-exchange chromatography and sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) prior to MS. This facilitated detection of a tryptic peptide map of CP4 EPSPS by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS and nanoelectrospray ionization quadrupole time-of-flight (nanoESI-QTOF) MS. Subsequently, sequence information from the CP4 EPSPS tryptic peptides was obtained by nanoESI-QTOF MS/MS. The identification was accomplished in 0.9% GM soya seeds, which is the current EU threshold for food-labeling requirements. Copyright © 2007 John Wiley & Sons, Ltd.