Laser-induced photofragmentation of neutral and acidic glycans inside an ion-trap mass spectrometer

Authors

  • Arugadoss Devakumar,

    1. National Center for Glycomics and Glycoproteomics and Department of Chemistry, Indiana University, Bloomington, IN 47405, USA
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  • Yehia Mechref,

    1. National Center for Glycomics and Glycoproteomics and Department of Chemistry, Indiana University, Bloomington, IN 47405, USA
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  • Pilsoo Kang,

    1. National Center for Glycomics and Glycoproteomics and Department of Chemistry, Indiana University, Bloomington, IN 47405, USA
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  • Milos V. Novotny,

    1. National Center for Glycomics and Glycoproteomics and Department of Chemistry, Indiana University, Bloomington, IN 47405, USA
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  • James P. Reilly

    Corresponding author
    1. National Center for Glycomics and Glycoproteomics and Department of Chemistry, Indiana University, Bloomington, IN 47405, USA
    • Department of Chemistry, Indiana University, 800 E Kirkwood Ave, Bloomington, IN 47405, USA.
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Abstract

Permethylated acidic and neutral N-glycans representing different types of glycan structures, such as linear and branched sialylated structures, high-mannose type and fucosylated complex type, were photodissociated with 157 nm vacuum ultraviolet light in a linear ion trap. Cross-ring fragments corresponding to high-energy fragmentation pathways were observed in abundance for all studied structures. Some product ions appear diagnostic for a linkage of sialic acid residues and the glycan antenna to which these residues are attached. A conclusive assignment of the fucosylation site of the studied glycan structure has been facilitated through measurement of cross-ring fragmentation resulting from photodissociation. Copyright © 2007 John Wiley & Sons, Ltd.

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