Multiresidue analysis of β-agonists in pork by coupling polymer monolith microextraction to electrospray quadrupole time-of-flight mass spectrometry
Article first published online: 31 JUL 2007
Copyright © 2007 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 21, Issue 17, pages 2895–2904, 15 September 2007
How to Cite
Huang, J.-F., Zhang, H.-J., Lin, B., Yu, Q.-W. and Feng, Y.-Q. (2007), Multiresidue analysis of β-agonists in pork by coupling polymer monolith microextraction to electrospray quadrupole time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom., 21: 2895–2904. doi: 10.1002/rcm.3165
- Issue published online: 31 JUL 2007
- Article first published online: 31 JUL 2007
- Manuscript Accepted: 29 JUN 2007
- Manuscript Revised: 28 JUN 2007
- Manuscript Received: 12 MAR 2007
- National Science Fund for Distinguished Young Scholars. Grant Number: 20625516
- Program for New Century Excellent Talents in University. Grant Number: NCET-05-0616
A novel method of polymer monolith microextraction (PMME) using poly(methacrylic acid-co-ethylene glycol dimethacrylate) monolith combined with electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QTOF MS) was developed for the rapid and sensitive determination of β-agonists in pork samples. The conditions of PMME were optimized for the improvement of extraction efficiency and reduction of the matrix interferences from pork. Under the optimal condition, the eluate solution allowed direct analysis by mass spectrometry. In the positive ion mode and in the multiple reaction monitoring (MRM) mode, the limits of detection (LODs) for β-agonists were found to be 0.08 ng/g (clenbuterol, CLB), 0.18 ng/g (salbutamol, SBTM) and 0.26 ng/g (terbutaline, TBTL) in pork, respectively, with good inter- and intra-day precisions (2–10% for CLB, 11–23% for SBTM and 4–16% for TBTL). The proposed PMME/ESI-QTOF MS method was successfully applied to the determination of β-agonist residues in thirteen real samples, and the positive samples were confirmed according to the identification points (IPs) system defined by Commission Decision 2002/657/EC. To investigate the matrix effect, the proposed method was compared with PMME-HPLC/ESI-QTOF MS and the slight decrease in sensitivity of PMME/ESI-QTOF MS was ascribed to the inter-analyte ion suppression. Copyright © 2007 John Wiley & Sons, Ltd.