Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids
Article first published online: 25 MAY 2001
Copyright © 2001 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 15, Issue 12, pages 969–974, 30 June 2001
How to Cite
Olsen, J. V., Haselmann, K. F., Nielsen, M. L., Budnik, B. A., Nielsen, P. E. and Zubarev, R. A. (2001), Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids. Rapid Commun. Mass Spectrom., 15: 969–974. doi: 10.1002/rcm.317
- Issue published online: 25 MAY 2001
- Article first published online: 25 MAY 2001
- Manuscript Accepted: 10 APR 2001
- Manuscript Revised: 9 APR 2001
- Manuscript Received: 12 MAR 2001
- Danish National Research Foundation. Grant Number: 51-00-0358
Electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry coupled with electrospray ionization enhances the sequence elucidation of peptide nucleic acids compared with conventional low-energy collisionally activated dissociation (CAD). Examples are shown where ECD produced complete or extensive sequence coverage in PNAs six to ten nucleobases long. However, facile base losses from the reduced species and low abundances of backbone ECD fragments presented a significant problem. This was rationalized through the lower degree of charge solvation on the backbone compared to polypeptides. Combination of both CAD and ECD data is advantageous, as these techniques produce cleavages at different sites. Copyright© 2001 John Wiley & Sons, Ltd.