Detection and quantification of sucrose as dietary biomarker using gas chromatography and liquid chromatography with mass spectrometry
Article first published online: 8 JAN 2008
Copyright © 2008 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 22, Issue 3, pages 279–282, 15 February 2008
How to Cite
Kuhnle, G. G. C., Joosen, A. M. C. P., Wood, T. R., Runswick, S. A., Griffin, J. L. and Bingham, S. A. (2008), Detection and quantification of sucrose as dietary biomarker using gas chromatography and liquid chromatography with mass spectrometry. Rapid Commun. Mass Spectrom., 22: 279–282. doi: 10.1002/rcm.3355
- Issue published online: 10 JAN 2008
- Article first published online: 8 JAN 2008
- Manuscript Accepted: 17 NOV 2007
- Manuscript Revised: 18 OCT 2007
- Manuscript Received: 7 SEP 2007
Several epidemiological studies suggest a link between the intake of refined sugars and an increased risk for colorectal, breast, pancreatic and endometrial cancer. However, other studies failed to confirm these conclusions and the reason for this may be the ambiguity of dietary assessment methods – mainly self-reporting – employed. Sucrose is an established biomarker for sugars intake, allowing the objective assessment of dietary sucrose. So far, urinary excretion of sucrose was mainly determined using an enzyme assay. However, this method is time-consuming and labour-intensive. In this study, we present a mass spectrometric method for the determination of sucrose in urine using liquid chromatography with mass spectrometry (LC/MS) which can be used for large-scale epidemiological studies. Copyright © 2008 John Wiley & Sons, Ltd.