Practical aspects of liquid chromatography/electrospray tandem mass spectrometry for rapid identification of metabolites of a new antifungal agent SYN-2836 in dog urine
Article first published online: 6 JUN 2001
Copyright © 2001 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 15, Issue 13, pages 1092–1100, 15 July 2001
How to Cite
Bu, H.-Z., Poglod, M., Micetich, R. G. and Khan, J. K. (2001), Practical aspects of liquid chromatography/electrospray tandem mass spectrometry for rapid identification of metabolites of a new antifungal agent SYN-2836 in dog urine. Rapid Commun. Mass Spectrom., 15: 1092–1100. doi: 10.1002/rcm.347
- Issue published online: 6 JUN 2001
- Article first published online: 6 JUN 2001
- Manuscript Accepted: 8 MAY 2001
- Manuscript Revised: 7 MAY 2001
- Manuscript Received: 23 MAR 2001
This report presents the structural elucidation of 12 urinary metabolites of SYN-2836, a new antifungal agent showing extensive metabolism in beagle dogs, using complementary liquid chromatography/tandem mass spectrometry (LC/MS/MS) methodologies. The 12 SYN-2836 metabolites were readily divided into four groups by considering that all three members of each group, although differing in masses, exhibited highly similar product ion mass spectra. This suggests that the metabolites within each group share a common major substructure. Therefore, all the grouped SYN-2836 metabolites were strategically identified by characterization of the major substructures followed by determination of the additional small substructures. This grouping strategy greatly facilitated the structural elucidation of these metabolites. Other strategies were also employed to achieve as rapid and unambiguous characterization of the SYN-2836 metabolites as possible. Copyright © 2001 John Wiley & Sons, Ltd.