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Practical aspects of liquid chromatography/electrospray tandem mass spectrometry for rapid identification of metabolites of a new antifungal agent SYN-2836 in dog urine

Authors

  • Hai-Zhi Bu,

    1. Division of Biopharmaceutics and Pharmacokinetics, NAEJA Pharmaceutical, Inc., 4290-91A Street, Edmonton, Alberta T6E 5V2, Canada
    Current affiliation:
    1. Department of Metabolic Chemistry, Covance Laboratories, Inc., Madison, WI 53704, USA
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  • Marzena Poglod,

    1. Division of Biopharmaceutics and Pharmacokinetics, NAEJA Pharmaceutical, Inc., 4290-91A Street, Edmonton, Alberta T6E 5V2, Canada
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  • Ronald G. Micetich,

    1. Division of Biopharmaceutics and Pharmacokinetics, NAEJA Pharmaceutical, Inc., 4290-91A Street, Edmonton, Alberta T6E 5V2, Canada
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  • Jehangir K. Khan

    Corresponding author
    1. Division of Biopharmaceutics and Pharmacokinetics, NAEJA Pharmaceutical, Inc., 4290-91A Street, Edmonton, Alberta T6E 5V2, Canada
    • Division of Biopharmaceutics and Pharmacokinetics, NAEJA Pharmaceutical, Inc., 4290-91A Street, Edmonton, Alberta T6E 5V2, Canada.
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Abstract

This report presents the structural elucidation of 12 urinary metabolites of SYN-2836, a new antifungal agent showing extensive metabolism in beagle dogs, using complementary liquid chromatography/tandem mass spectrometry (LC/MS/MS) methodologies. The 12 SYN-2836 metabolites were readily divided into four groups by considering that all three members of each group, although differing in masses, exhibited highly similar product ion mass spectra. This suggests that the metabolites within each group share a common major substructure. Therefore, all the grouped SYN-2836 metabolites were strategically identified by characterization of the major substructures followed by determination of the additional small substructures. This grouping strategy greatly facilitated the structural elucidation of these metabolites. Other strategies were also employed to achieve as rapid and unambiguous characterization of the SYN-2836 metabolites as possible. Copyright © 2001 John Wiley & Sons, Ltd.

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