A rapid and precise method for determination of D/H ratios of plant methoxyl groups
Article first published online: 13 NOV 2008
Copyright © 2008 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 22, Issue 24, pages 3983–3988, 30 December 2008
How to Cite
Greule, M., Mosandl, A., Hamilton, J. T. G. and Keppler, F. (2008), A rapid and precise method for determination of D/H ratios of plant methoxyl groups. Rapid Commun. Mass Spectrom., 22: 3983–3988. doi: 10.1002/rcm.3817
- Issue published online: 13 NOV 2008
- Article first published online: 13 NOV 2008
- Manuscript Revised: 2 OCT 2008
- Manuscript Accepted: 2 OCT 2008
- Manuscript Received: 11 AUG 2008
- The European Science Foundation (ESF) and the German Science Foundation (DFG)
Stable hydrogen isotope ratio measurements of specific plant components are increasingly used in numerous fields of research, including sample origin verification and climate research. A recently suggested method with considerable potential in this context is the D/H isotope ratio (δ2H value) analysis of plant matter methoxyl groups. The method entails ether or ester cleavage with hydriodic acid (HI) to form the gaseous compound methyl iodide (CH3I) and measurement of the δ2H value of this gas. Here we describe a method for the rapid and precise δ2H analysis of plant matter methoxyl groups using gas chromatography/pyrolysis/isotope ratio mass spectrometry (GC/P/IRMS). The conditions for sample preparation were investigated for isotope discrimination effects, the GC conditions were optimized, the reproducibility of the measurement of standards was studied, and the precision of the method was defined. The reproducibility of δ2H values determined for a CH3I standard on 20 consecutive measurements was found to be 2‰. The method was also tested on four methoxyl-rich plant components: vanillin, lignin, wood and pectin. The analytical precision obtained, when expressed as the average standard deviation for these compounds, was better than 1.6‰. The described method is rapid, allowing preparation and analysis of a sample within 1 h, and produces accurate and reproducible isotopic measurements. Copyright © 2008 John Wiley & Sons, Ltd.