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Profiling the metabolic differences of anthraquinone derivatives using liquid chromatography/tandem mass spectrometry with data-dependent acquisition

Authors

  • Rui Song,

    1. Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, P.R. China
    2. Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, P.R. China
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  • Hui Lin,

    1. Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, P.R. China
    2. Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, P.R. China
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  • Zunjian Zhang,

    Corresponding author
    1. Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, P.R. China
    2. Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, P.R. China
    • Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, P.R. China.
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  • Zhe Li,

    1. Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, P.R. China
    2. Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, P.R. China
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  • Lei Xu,

    1. Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, P.R. China
    2. Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, P.R. China
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  • Haijuan Dong,

    1. Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, P.R. China
    2. Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, P.R. China
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  • Yuan Tian

    1. Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, P.R. China
    2. Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, P.R. China
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Abstract

This work concentrates on the invitro metabolism of anthraquinone derivatives from rhubarb. Different metabolic reactions for anthraquinone derivatives were dependent on the substituent group in the skeleton. Monohydroxylation in the exocyclic methyl group was the major metabolic modification for emodin. Aloe-emodin containing a hydroxymethyl group was mainly metabolized through a methylation reaction. For rhein, both hydrogenation and methyl substitution on the benzene ring were the major metabolic reactions. Hydroxyl substitution on the benzene ring was the predominant metabolic reaction for chrysophanol. For physcion, demethylation of the exocyclic methoxy group was the most important metabolic reaction. Copyright © 2009 John Wiley & Sons, Ltd.

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