Evaluating uncertainty in the calculation of non-exchangeable hydrogen fractions within organic materials
Article first published online: 20 MAR 2009
Copyright © 2009 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 23, Issue 9, pages 1275–1280, 15 May 2009
How to Cite
Chesson, L. A., Podlesak, D. W., Cerling, T. E. and Ehleringer, J. R. (2009), Evaluating uncertainty in the calculation of non-exchangeable hydrogen fractions within organic materials. Rapid Commun. Mass Spectrom., 23: 1275–1280. doi: 10.1002/rcm.4000
- Issue published online: 20 MAR 2009
- Article first published online: 20 MAR 2009
- Manuscript Accepted: 25 FEB 2009
- Manuscript Revised: 24 FEB 2009
- Manuscript Received: 31 DEC 2008
- IsoForensics Inc.
We calculated the fraction of exchangeable hydrogen atoms in proteinaceous materials commonly analyzed for stable isotopic composition related to the region-of-origin of an animal. These included several types of α- and β-keratin, and muscle tissue. We find that the fraction of H atoms in keratin available for exchange at a biologically relevant temperature (25°C) averaged 9% across a range of ground organic materials, but was as high as ∼17% in cut hair; muscle tissue has ∼12% exchangeable H atoms. Under most analysis conditions, the difference in exchangeable fractions due to physical sample processing has a minimal effect on the calculated δ2H values of the non-exchangeable H atoms within a keratin-containing tissue (<2‰). However, extreme mismatches between sample and reference material types could affect δ2H values. Copyright © 2009 John Wiley & Sons, Ltd.