Mass spectrometry of intact neutral macromolecules using intense non-resonant femtosecond laser vaporization with electrospray post-ionization
Article first published online: 27 AUG 2009
Copyright © 2009 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 23, Issue 19, pages 3151–3157, 15 October 2009
How to Cite
Brady, J. J., Judge, E. J. and Levis, R. J. (2009), Mass spectrometry of intact neutral macromolecules using intense non-resonant femtosecond laser vaporization with electrospray post-ionization. Rapid Commun. Mass Spectrom., 23: 3151–3157. doi: 10.1002/rcm.4226
- Issue published online: 27 AUG 2009
- Article first published online: 27 AUG 2009
- Manuscript Accepted: 23 JUL 2009
- Manuscript Revised: 22 JUL 2009
- Manuscript Received: 10 JUN 2009
Intact, nonvolatile, biological macromolecules can be transferred directly from the solid state into the gas phase, in ambient air, for subsequent mass spectral analysis using non-resonant femtosecond (fs) laser desorption combined with electrospray ionization (ESI). Mass spectral measurements for neat samples, including a dipeptide, protoporphyrin IX and vitamin B12 adsorbed on a glass insulating surface, were obtained using an 800 nm, 70 fs laser with an intensity of 1013 W cm−2. No appreciable signal was detected when atmospheric matrix-assisted or neat (matrix-free) fs laser desorption was performed without ESI, indicating neutral desorption. Copyright © 2009 John Wiley & Sons, Ltd.