Matrix-assisted laser desorption/ionization imaging mass spectrometry of oxaliplatin derivatives in heated intraoperative chemotherapy (HIPEC)-like treated rat kidney

Authors

  • Amina Bouslimani,

    1. IRCM, Institut de Recherche en Cancérologie de Montpellier, F-34298 Montpellier, France; INSERM, U896, F-34298 Montpellier, France; Université Montpellier 1, F-34298 Montpellier, France; CRLC Val d'Aurelle Paul Lamarque, F-34298 Montpellier, France
    2. Oncopharmacology Department, Pharmacy Service, CRLC Val d'Aurelle Paul Lamarque, F-34298 Montpellier, France
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  • Nicole Bec,

    1. IRCM, Institut de Recherche en Cancérologie de Montpellier, F-34298 Montpellier, France; INSERM, U896, F-34298 Montpellier, France; Université Montpellier 1, F-34298 Montpellier, France; CRLC Val d'Aurelle Paul Lamarque, F-34298 Montpellier, France
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  • Matthias Glueckmann,

    1. Applied Biosystems, Frankfurter Str. 129b, D-64293 Darmstadt, Germany
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  • Christophe Hirtz,

    1. IRCM, Institut de Recherche en Cancérologie de Montpellier, F-34298 Montpellier, France; INSERM, U896, F-34298 Montpellier, France; Université Montpellier 1, F-34298 Montpellier, France; CRLC Val d'Aurelle Paul Lamarque, F-34298 Montpellier, France
    2. EA 4203, UFR Odontologie, Université Montpellier I, 34193 Montpellier Cedex 5, France
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  • Christian Larroque

    Corresponding author
    1. IRCM, Institut de Recherche en Cancérologie de Montpellier, F-34298 Montpellier, France; INSERM, U896, F-34298 Montpellier, France; Université Montpellier 1, F-34298 Montpellier, France; CRLC Val d'Aurelle Paul Lamarque, F-34298 Montpellier, France
    • IRCM, Institut de Recherche en Cancérologie de Montpellier, F-34298 Montpellier, France; INSERM, U896, F-34298 Montpellier, France; Université Montpellier 1, F-34298 Montpellier, France; CRLC Val d'Aurelle Paul Lamarque, F-34298 Montpellier, France.
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Abstract

Oxaliplatin [1,2-diaminocyclohexane (dach)-Pt complex] is a platinum anticancer drug which is mainly used in the treatment of advanced colorectal cancer, particularly in Heated Intraoperative Chemotherapy (HIPEC) for the treatment of colorectal peritoneal carcinomatosis. In order to better understand the penetration of oxaliplatin in treated tissues we performed a direct imaging of tissue sections from HIPEC-like treated rat kidney using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. This procedure allowed the detection and localization of oxaliplatin and its metabolites, the monocysteine and monomethionine complexes, in kidney sections. Specifically, oxaliplatin and its metabolites were localized exclusively in the kidney cortex, suggesting that it did not penetrate deeply into the organ. Based on these results, an imaging analysis of human tumors collected after HIPEC is currently in progress to assess the distribution of oxaliplatin and/or metabolites with the aim of defining clinical conditions to improve drug penetration. Copyright © 2010 John Wiley & Sons, Ltd.

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