In situ probing of cholesterol in astrocytes at the single-cell level using laser desorption ionization mass spectrometric imaging with colloidal silver

Authors

  • D. C. Perdian,

    1. Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, IA 50011, USA
    2. Department of Chemistry, Iowa State University, Ames, IA 50011, USA
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    • These authors contributed equally to this work.

  • Sangwon Cha,

    1. Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, IA 50011, USA
    2. Department of Chemistry, Iowa State University, Ames, IA 50011, USA
    Current affiliation:
    1. The Barnett Institute, Northeastern University, Boston, MA 02115, USA.
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    • These authors contributed equally to this work.

  • Jisun Oh,

    1. Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA
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  • Donald S. Sakaguchi,

    1. Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA
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  • Edward S. Yeung,

    1. Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, IA 50011, USA
    2. Department of Chemistry, Iowa State University, Ames, IA 50011, USA
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  • Young Jin Lee

    Corresponding author
    1. Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, IA 50011, USA
    2. Department of Chemistry, Iowa State University, Ames, IA 50011, USA
    • Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA 50011, USA.
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  • This article is a U.S. Government work and is in the public domain in the U.S.A.

Abstract

Mass spectrometric imaging has been utilized to localize individual astrocytes and to obtain cholesterol populations at the single-cell level in laser desorption ionization (LDI) with colloidal silver. The silver ion adduct of membrane-bound cholesterol was monitored to detect individual cells. Good correlation between mass spectrometric and optical images at different cell densities indicates the ability to perform single-cell studies of cholesterol abundance. The feasibility of quantification is confirmed by the agreement between the LDI-MS ion signals and the results from a traditional enzymatic fluorometric assay. We propose that this approach could be an effective tool to study chemical populations at the cellular level. Published in 2010 by John Wiley & Sons, Ltd.

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