Structural identification of mouse urinary metabolites of pterostilbene using liquid chromatography/tandem mass spectrometry
Article first published online: 17 MAY 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 24, Issue 12, pages 1770–1778, 30 June 2010
How to Cite
Shao, X., Chen, X., Badmaev, V., Ho, C.-T. and Sang, S. (2010), Structural identification of mouse urinary metabolites of pterostilbene using liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom., 24: 1770–1778. doi: 10.1002/rcm.4579
- Issue published online: 19 MAY 2010
- Article first published online: 17 MAY 2010
- Manuscript Accepted: 14 APR 2010
- Manuscript Revised: 11 APR 2010
- Manuscript Received: 8 JAN 2010
Pterostilbene, the dimethoxy derivative of resveratrol, has drawn much attention recently due to its potential beneficial health effects. The metabolic fate of pterostilbene, however, is not well understood. In the present study, we identified nine novel mouse urinary pterostilbene metabolites, pterostilbene glucuronide, pterostilbene sulfate, mono-demethylated pterostilbene glucuronide, mono-demethylated pterostilbene sulfate, mono-hydroxylated pterostilbene, mono-hydroxylated pterostilbene glucuronide, mono-hydroxylated pterostilbene sulfate, and mono-hydroxylated pterostilbene glucuronide sulfate, using liquid chromatography/atmospheric pressure chemical ionization and electrospray ionization tandem mass spectrometry. The structures of these metabolites were confirmed by analyzing the MSn (n = 1–3) spectra. To our knowledge, this is the first report of the identification of urinary metabolites of pterostilbene in mice. Copyright © 2010 John Wiley & Sons, Ltd.