Structural identification of mouse urinary metabolites of pterostilbene using liquid chromatography/tandem mass spectrometry

Authors

  • Xi Shao,

    1. Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA
    2. Human Nutrition Research Program, Julius L. Chambers Biomedical/Biotechnology Research Institute, North Carolina Central University; North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA
    3. Food Science Graduate Program, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA
    4. Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA
    Search for more papers by this author
  • Xiaoxin Chen,

    1. Cancer Research Program, Julius L. Chambers Biomedical/Biotechnology Research Institute, North Carolina Central University, 700 George Street, Durham, NC 27707, USA
    Search for more papers by this author
  • Vladimir Badmaev,

    1. Laboratory of Applied Pharmacology, 1440-6 Forest Hill Road, Staten Island, NY 10314, USA
    Search for more papers by this author
  • Chi-Tang Ho,

    1. Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA
    Search for more papers by this author
  • Shengmin Sang

    Corresponding author
    1. Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA
    2. Human Nutrition Research Program, Julius L. Chambers Biomedical/Biotechnology Research Institute, North Carolina Central University; North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA
    3. Food Science Graduate Program, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA
    • Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC 28081, USA.
    Search for more papers by this author

Abstract

Pterostilbene, the dimethoxy derivative of resveratrol, has drawn much attention recently due to its potential beneficial health effects. The metabolic fate of pterostilbene, however, is not well understood. In the present study, we identified nine novel mouse urinary pterostilbene metabolites, pterostilbene glucuronide, pterostilbene sulfate, mono-demethylated pterostilbene glucuronide, mono-demethylated pterostilbene sulfate, mono-hydroxylated pterostilbene, mono-hydroxylated pterostilbene glucuronide, mono-hydroxylated pterostilbene sulfate, and mono-hydroxylated pterostilbene glucuronide sulfate, using liquid chromatography/atmospheric pressure chemical ionization and electrospray ionization tandem mass spectrometry. The structures of these metabolites were confirmed by analyzing the MSn (n = 1–3) spectra. To our knowledge, this is the first report of the identification of urinary metabolites of pterostilbene in mice. Copyright © 2010 John Wiley & Sons, Ltd.

Ancillary