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Direct analysis of cellulose in poplar stem by matrix-assisted laser desorption/ionization imaging mass spectrometry

Authors

  • Seokwon Jung,

    1. BioEnergy Science Center, Georgia Institute of Technology, 500 10th St., Atlanta, GA 30332, USA
    2. School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332, USA
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  • Yanfeng Chen,

    1. School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332, USA
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  • M. Cameron Sullards,

    1. BioEnergy Science Center, Georgia Institute of Technology, 500 10th St., Atlanta, GA 30332, USA
    2. School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332, USA
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  • Arthur J. Ragauskas

    Corresponding author
    1. BioEnergy Science Center, Georgia Institute of Technology, 500 10th St., Atlanta, GA 30332, USA
    2. Institute of Paper Science and Technology, Georgia Institute of Technology, 500 10th St., Atlanta, GA 30332, USA
    3. School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332, USA
    • BioEnergy Science Center, Georgia Institute of Technology, 500 10th St., Atlanta, GA 30332, USA.
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Abstract

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) was applied to the analysis of the spatial distribution of cellulose on a cross-section of juvenile poplar (Populus deltoids) stems. Microcrystalline cellulose (MCC) was used to optimize matrix (2,5-dihydroxybenzoic acid) application and instrument parameters for the detection of low hexose oligomers, which originated from cellulose in the solid phase. A section of poplar cellulose isolated from juvenile poplar stem which consisted primarily of glucose (∼95%) and minor components such as xylose and lignin was used for the MALDI-IMS studies. The mass spectrum of poplar cellulose consisted of a series of evenly spaced signals having a difference of 162 m/z units, which was similar to that of MCC in linear and reflectron positive ion modes. MS images of cellulose compounds with sodium ion adducts were generated and illustrated the distribution of cellulose on the surface of the poplar stem. Copyright © 2010 John Wiley & Sons, Ltd.

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