Binding of alpha 1-acid glycoprotein with aconitum alkaloids: an investigation using an intensity fading matrix-assisted laser desorption/ionization Fourier transform mass spectrometry method
Article first published online: 14 MAR 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 25, Issue 7, pages 973–978, 15 April 2011
How to Cite
Liu, W., Liu, S., Li, H., Song, F., Liu, Z. and Liu, S. (2011), Binding of alpha 1-acid glycoprotein with aconitum alkaloids: an investigation using an intensity fading matrix-assisted laser desorption/ionization Fourier transform mass spectrometry method. Rapid Commun. Mass Spectrom., 25: 973–978. doi: 10.1002/rcm.4947
- Issue published online: 17 MAR 2011
- Article first published online: 14 MAR 2011
- Manuscript Accepted: 13 JAN 2011
- Manuscript Revised: 22 DEC 2010
- Manuscript Received: 27 JUL 2010
- National Natural Science Foundation of China. Grant Numbers: 81073034, 30772721
- Major State Basic Research Development Program of China (973 Program). Grant Number: 2006CB5047060
Intensity fading matrix-assisted laser desorption/ionization (IF-MALDI) mass spectrometry has become an alternative screening approach for the affinity-binding analysis of proteins and peptides with ligands. In this study, an attempt has been made to study the interaction of alpha 1-acid glycoprotein (AGP) with aconitum alkaloids by IF-MALDI Fourier transform ion cyclotron resonance mass spectrometry (IF-MALDI-FT-MS). Compared with the nonbinding internal standard, clear reduction in the ion abundances of the target alkaloids was observed with the addition of AGP. Relative binding affinities of different alkaloids towards the protein could also be estimated using IF-MALDI-FT-MS. The binding affinity was also investigated by using ultrafiltration liquid chromatography with photodiode array detection coupled to electrospray ionization mass spectrometry (ultrafiltration LC-DAD/ESI-MS), and results were consistent with that of IF-MALDI-FT-MS. Copyright © 2011 John Wiley & Sons, Ltd.