Electrospray ionization with ambient pressure ion mobility separation and mass analysis by orthogonal time-of-flight mass spectrometry
Article first published online: 29 OCT 2001
Copyright © 2001 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 15, Issue 23, pages 2221–2226, 15 December 2001
How to Cite
Steiner, W. E., Clowers, B. H., Fuhrer, K., Gonin, M., Matz, L. M., Siems, W. F., Schultz, A. J. and Hill, H. H. (2001), Electrospray ionization with ambient pressure ion mobility separation and mass analysis by orthogonal time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom., 15: 2221–2226. doi: 10.1002/rcm.495
- Issue published online: 29 OCT 2001
- Article first published online: 29 OCT 2001
- Manuscript Accepted: 28 SEP 2001
- Manuscript Revised: 27 SEP 2001
- Manuscript Received: 1 AUG 2001
- US Army Research Office. Grant Number: DAAG 559810107 and DAAD 190010028
Rapid screening and identification of drug and other mixtures are possible using a novel ambient pressure high-resolution ion mobility (APIMS) orthogonal reflector time-of-flight mass spectrometer (TOFMS). Departing ions from the APIMS drift tube traversed a pressure interface between the APIMS and TOFMS where they were subjected to numerous gas collisions that could produce selective fragmentation. By increasing the accelerating field in the pressure interface region, the ions generated using water-cooled electrospray ionization (ESI) underwent collision-induced dissociation (CID). Mixtures of ESI ions were separated by APIMS based on their respective size-to-charge (s/z) ratios while CID and analysis of mass-to-charge (m/z) ratios occurred in the pressure interface and TOFMS. Product ions that were formed in this pressure interface region could be readily assigned to precursor ions by matching the mobility drift times. This process was demonstrated by the examination of a mixture of amphetamines and the resulting fragmentation patterns of the mobility-separated precursor ion species [M + H]+. Copyright © 2001 John Wiley & Sons, Ltd.