Fast screening of highly glycosylated plant sphingolipids by tandem mass spectrometry

Authors

  • Corinne Buré,

    Corresponding author
    • Université de Bordeaux, Chimie Biologie des Membranes et Nanoobjets CBMN – UMR 5248, Centre de Génomique Fonctionnelle Université Bordeaux 2, Bordeaux Cedex, France
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  • Jean-Luc Cacas,

    1. Université de Bordeaux, Laboratoire de Biogenèse Membranaire, UMR 5200 CNRS-Université Bordeaux Segalen, Bordeaux cedex, France
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  • Fen Wang,

    1. Université de Bordeaux, Laboratoire de Biogenèse Membranaire, UMR 5200 CNRS-Université Bordeaux Segalen, Bordeaux cedex, France
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  • Karen Gaudin,

    1. EA 4575 Analytical and Pharmaceutical Developments applied to Neglected Diseases and Counterfeit Drugs, Université Bordeaux Segalen, Bordeaux cedex, France
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  • Frédéric Domergue,

    1. Université de Bordeaux, Laboratoire de Biogenèse Membranaire, UMR 5200 CNRS-Université Bordeaux Segalen, Bordeaux cedex, France
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  • Sébastien Mongrand,

    1. Université de Bordeaux, Laboratoire de Biogenèse Membranaire, UMR 5200 CNRS-Université Bordeaux Segalen, Bordeaux cedex, France
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  • Jean-Marie Schmitter

    1. Université de Bordeaux, Chimie Biologie des Membranes et Nanoobjets CBMN – UMR 5248, Centre de Génomique Fonctionnelle Université Bordeaux 2, Bordeaux Cedex, France
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C. Buré, Chimie Biologie des Membranes et Nanoobjets CBMN – UMR 5248 Centre de Génomique Fonctionnelle BP 68, Université Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France.

E-mail: c.bure@cbmn.u-bordeaux.fr

Abstract

The structural characterization of Glycosyl-Inositol-Phospho-Ceramides (GIPCs), which are the main sphingolipids of plant tissues, is a critical step towards the understanding of their physiological function. After optimization of their extraction, numerous plant GIPCs have been characterized by mass spectrometry. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) full scan analysis of negative ions provides a quick overview of GIPC distribution. Clear differences were observed for the two plant models studied: six GIPC series bearing from two to seven saccharide units were detected in tobacco BY-2 cell extracts, whereas GIPCs extracted from A. thaliana cell cultures and leaves were less diverse, with a dominance of species containing only two saccharide units. The number of GIPC species was around 50 in A. thaliana and 120 in tobacco BY-2 cells. MALDI-MS/MS spectra gave access to detailed structural information relative to the ceramide moiety, the polar head, as well as the number and types of saccharide units. Once released from GIPCs, fatty acid chains and long-chain bases were analyzed by GC/MS to verify that all GIPC series were taken into account by the MALDI-MS/MS approach. ESI-MS/MS provided complementary information for the identification of isobaric species and fatty acid chains. Such a methodology, mostly relying on MALDI-MS/MS, should open new avenues to determine structure-function relationships between glycosphingolipids and membrane organization. Copyright © 2011 John Wiley & Sons, Ltd.

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