Separation of peptides from detergents using ion mobility spectrometry
Article first published online: 12 OCT 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 25, Issue 22, pages 3436–3440, 30 November 2011
How to Cite
Bagag, A., Giuliani, A., Canon, F., Réfrégiers, M. and Naour, F. L. (2011), Separation of peptides from detergents using ion mobility spectrometry. Rapid Commun. Mass Spectrom., 25: 3436–3440. doi: 10.1002/rcm.5242
- Issue published online: 10 OCT 2011
- Article first published online: 12 OCT 2011
- Manuscript Revised: 6 SEP 2011
- Manuscript Accepted: 6 SEP 2011
- Manuscript Received: 16 JUN 2011
Mass spectrometry (MS) has dramatically evolved in the last two decades and has been the driving force of the spectacular expansion of proteomics during this period. However, the very poor compatibility of MS with detergents is still a technical obstacle in some studies, in particular on membrane proteins. Indeed, the high hydrophobicity of membrane proteins necessitates the use of detergents for their extraction and solubilization. Here, we address the analytical potential of high-field asymmetric waveform ion mobility spectrometry (FAIMS) for separating peptides from detergents. The study was focused on peptides from the human integral membrane protein CD9. A tryptic peptide was mixed with the non-ionic detergents Triton X-100 or beta-D-dodecyl maltoside (DDM) as well as with the ionic detergents sodium dodecyl sulfate (SDS) or sodium deoxycholate (SDC). Although electrospray ionization (ESI) alone led to a total suppression of the peptide ion signal on mass spectra with only detection of the detergents, use of FAIMS allowed separation and clear identification of the peptide with any of the detergents studied. The detection and identification of the target compound in the presence of an excess of detergents are then feasible. FAIMS should prove especially useful in the structural and proteomic analysis of membrane proteins. Copyright © 2011 John Wiley & Sons, Ltd.