Characterization and identification of chemical compositions in the extract of Artemisia rupestris L. by liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry
Article first published online: 2 DEC 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 26, Issue 1, pages 83–100, 15 January 2012
How to Cite
Gu, D., Yang, Y., Abdulla, R. and Aisa, H. A. (2012), Characterization and identification of chemical compositions in the extract of Artemisia rupestris L. by liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry. Rapid Commun. Mass Spectrom., 26: 83–100. doi: 10.1002/rcm.5289
- Issue published online: 2 DEC 2011
- Article first published online: 2 DEC 2011
- Manuscript Accepted: 10 OCT 2011
- Manuscript Revised: 9 OCT 2011
- Manuscript Received: 15 AUG 2011
Liquid chromatography coupled to negative electrospray ionization (ESI) tandem mass spectrometry (MS/MS) employing a time-of-flight tandem mass spectrometer was used in the structural determination of phenolic compounds and sesquiterpenoids occurring in the extract from Artemisia rupestris L. A total of 91 compounds including chlorogenic acid derivatives, flavonoids (aglycone, O-glycosyl, C-glycosyl and C,O-glycosyl), 2-phenoxychromones and guaiane sesquiterpenoids were identified by comparing the retention time and fragmentation behavior with reference standards or according to accurate mass measurement and the characteristic fragmentation at low and high collision energy. Most of these compounds were reported in Artemisia rupestris L. for the first time. Meanwhile, the proposed pathway and the major diagnostic fragmentation of 2-phenoxychromone and rupestonic acid were investigated to trace 2-phenoxychromone and rupestonic acid derivatives in crude plant extracts. According to these rules, we have successfully characterized five potential novel compounds including three 2-phenoxychromones (6-demethoxy-4′-O-methylcapillarisin-O-hexosylglucuronide, 6-demethoxy-4′-O-methylcapillarisin-O-pentosylhexoside and 6-demethoxy-4′-O-methylcapillarisin-O-deoxyhexosylhexoside) and two sesquiterpenoids (hexosyl-glycurinide-rupestonic acid and hexoside-rupestonic acid). Copyright © 2011 John Wiley & Sons, Ltd.