Research Article

High-performance thin-layer chromatography plate blotting for liquid microjunction surface sampling probe mass spectrometric analysis of analytes separated on a wettable phase plate†

  1. Matthew J. Walworth1,2,‡,
  2. Joseph J. Stankovich1,2,§,
  3. Gary J. Van Berkel1,*,
  4. Michael Schulz3,
  5. Susanne Minarik3

Article first published online: 2 DEC 2011

DOI: 10.1002/rcm.5299

Issue

Rapid Communications in Mass Spectrometry

Rapid Communications in Mass Spectrometry

Volume 26, Issue 1, pages 37–42, 15 January 2012

Additional Information

How to Cite

Walworth, M. J., Stankovich, J. J., Van Berkel, G. J., Schulz, M. and Minarik, S. (2012), High-performance thin-layer chromatography plate blotting for liquid microjunction surface sampling probe mass spectrometric analysis of analytes separated on a wettable phase plate. Rapid Commun. Mass Spectrom., 26: 37–42. doi: 10.1002/rcm.5299

Author Information

  1. 1

    Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA

  2. 2

    Department of Chemistry, University of Tennessee, Knoxville, Tennessee, USA

  3. 3

    Thin-Layer Chromatography Laboratory, Merck Millipore – Lab Essentials, Merck KGaA, Darmstadt, Germany

  1. ‡

    Current address: Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, IN 46285, USA.

  2. §

    Current address: Department of Chemistry, University of Tennessee, Knoxville, Tennessee 37996-1600, USA.

*G. J. Van Berkel, Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge, National Laboratory, Oak Ridge, TN 37831-6131, USA.
E-mail: vanberkelgj@ornl.gov

  • ‡

    Current address: Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, IN 46285, USA.

  • §

    Current address: Department of Chemistry, University of Tennessee, Knoxville, Tennessee 37996-1600, USA.

  • †

    This article is a U.S. Government work and is in the public domain in the U.S.A.

Publication History

  1. Issue published online: 2 DEC 2011
  2. Article first published online: 2 DEC 2011
  3. Manuscript Accepted: 15 OCT 2011
  4. Manuscript Revised: 14 OCT 2011
  5. Manuscript Received: 7 SEP 2011

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A blotting method that transfers analytes separated on wettable high-performance thin-layer chromatography (HPTLC) plates to a hydrophobic reversed-phase C8 HPLTC plate suitable for analysis with a liquid microjunction surface sampling probe electrospray ionization mass spectrometry system was described and demonstrated. The simple blotting procedure transfers the analyte from the wettable plate to the topmost surface of a rigidly backed, easy-to-mount hydrophobic substrate that already has been proven viable for analysis by this sampling probe/mass spectrometry system. The utility of the approach was demonstrated by the analysis of a four-component peptide mixture originally separated on a ProteoChrom® HPTLC cellulose sheet and then blotted onto the reversed-phase HPTLC plate. Published in 2011 by John Wiley & Sons, Ltd.

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