Current address: Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, IN 46285, USA.
High-performance thin-layer chromatography plate blotting for liquid microjunction surface sampling probe mass spectrometric analysis of analytes separated on a wettable phase plate†
Article first published online: 2 DEC 2011
Published in 2011 by John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 26, Issue 1, pages 37–42, 15 January 2012
How to Cite
Walworth, M. J., Stankovich, J. J., Van Berkel, G. J., Schulz, M. and Minarik, S. (2012), High-performance thin-layer chromatography plate blotting for liquid microjunction surface sampling probe mass spectrometric analysis of analytes separated on a wettable phase plate. Rapid Commun. Mass Spectrom., 26: 37–42. doi: 10.1002/rcm.5299
This article is a U.S. Government work and is in the public domain in the U.S.A.
- Issue published online: 2 DEC 2011
- Article first published online: 2 DEC 2011
- Manuscript Accepted: 15 OCT 2011
- Manuscript Revised: 14 OCT 2011
- Manuscript Received: 7 SEP 2011
A blotting method that transfers analytes separated on wettable high-performance thin-layer chromatography (HPTLC) plates to a hydrophobic reversed-phase C8 HPLTC plate suitable for analysis with a liquid microjunction surface sampling probe electrospray ionization mass spectrometry system was described and demonstrated. The simple blotting procedure transfers the analyte from the wettable plate to the topmost surface of a rigidly backed, easy-to-mount hydrophobic substrate that already has been proven viable for analysis by this sampling probe/mass spectrometry system. The utility of the approach was demonstrated by the analysis of a four-component peptide mixture originally separated on a ProteoChrom® HPTLC cellulose sheet and then blotted onto the reversed-phase HPTLC plate. Published in 2011 by John Wiley & Sons, Ltd.