High-performance thin-layer chromatography plate blotting for liquid microjunction surface sampling probe mass spectrometric analysis of analytes separated on a wettable phase plate$

Authors

  • Matthew J. Walworth,

    1. Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA
    2. Department of Chemistry, University of Tennessee, Knoxville, Tennessee, USA
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    • Current address: Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, IN 46285, USA.

  • Joseph J. Stankovich,

    1. Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA
    2. Department of Chemistry, University of Tennessee, Knoxville, Tennessee, USA
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    • Current address: Department of Chemistry, University of Tennessee, Knoxville, Tennessee 37996-1600, USA.

  • Gary J. Van Berkel,

    Corresponding author
    • Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA
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  • Michael Schulz,

    1. Thin-Layer Chromatography Laboratory, Merck Millipore – Lab Essentials, Merck KGaA, Darmstadt, Germany
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  • Susanne Minarik

    1. Thin-Layer Chromatography Laboratory, Merck Millipore – Lab Essentials, Merck KGaA, Darmstadt, Germany
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  • $

    This article is a U.S. Government work and is in the public domain in the U.S.A.

G. J. Van Berkel, Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge, National Laboratory, Oak Ridge, TN 37831-6131, USA.

E-mail: vanberkelgj@ornl.gov

Abstract

A blotting method that transfers analytes separated on wettable high-performance thin-layer chromatography (HPTLC) plates to a hydrophobic reversed-phase C8 HPLTC plate suitable for analysis with a liquid microjunction surface sampling probe electrospray ionization mass spectrometry system was described and demonstrated. The simple blotting procedure transfers the analyte from the wettable plate to the topmost surface of a rigidly backed, easy-to-mount hydrophobic substrate that already has been proven viable for analysis by this sampling probe/mass spectrometry system. The utility of the approach was demonstrated by the analysis of a four-component peptide mixture originally separated on a ProteoChrom® HPTLC cellulose sheet and then blotted onto the reversed-phase HPTLC plate. Published in 2011 by John Wiley & Sons, Ltd.

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