Studies in search of selective detection of isomeric biogenic hexen-1-ols and hexanal by flowing afterglow tandem mass spectrometry using [H3O]+ and [NO]+ reagent ions
Article first published online: 10 JUL 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 26, Issue 16, pages 1868–1874, 30 August 2012
How to Cite
Dhooghe, F., Vansintjan, R., Schoon, N. and Amelynck, C. (2012), Studies in search of selective detection of isomeric biogenic hexen-1-ols and hexanal by flowing afterglow tandem mass spectrometry using [H3O]+ and [NO]+ reagent ions. Rapid Commun. Mass Spectrom., 26: 1868–1874. doi: 10.1002/rcm.6294
- Issue published online: 2 JUL 2012
- Article first published online: 10 JUL 2012
- Manuscript Revised: 22 MAY 2012
- Manuscript Accepted: 22 MAY 2012
- Manuscript Received: 8 FEB 2012
Plants emit a blend of oxygenated volatile C6 compounds, known as green leaf volatiles (GLVs), in response to leaf tissue damage related to stress conditions. On-line chemical ionization mass spectrometry (CI-MS) techniques have often been used to study the dynamics of these emissions but they fail to selectively detect some important GLV compounds.
A flowing afterglow tandem mass spectrometer (FA-TMS) was used to investigate the feasibility of selective on-line detection of isomeric hexen-1-ols and hexanal. Product ions at m/z 101 and 83 from chemical ionization (CI) of these compounds by [H3O]+, and product ions at m/z 100, 99, 83, 82 and 72 from CI by [NO]+, have been subjected to collision-induced dissociation (CID) in the collision cell of the TMS at center-of-mass energies ranging between 0 and 9 eV.
CID of product ions at m/z 101 and 83 from CI of GLVs with [H3O]+ and of product ions at m/z 83, 82 and 72 from CI of GLVs with [NO]+ resulted in identical fragmentation patterns for all measured compounds, ruling out any selectivity. However, CID of product ions at m/z 100 and 99 from CI by [NO]+ led to CID product ions with abundances differing largely between the compounds, allowing the fast selective detection of 2-hexen-1-ols, 3-hexen-1-ols and hexanal with a chosen accuracy within a well-defined range of relative concentrations.
This research illustrates that, in contrast to common CI-MS techniques, FA-TMS allows the selective detection of hexanal in a mixture of hexanal and hexen-1-ols with a chosen accuracy for a well-defined range of relative concentrations and represents a step forward in the search for selective detection of GLVs in CI-TMS. Copyright © 2012 John Wiley & Sons, Ltd.