Matrix-assisted laser desorption/ionization imaging mass spectrometry of pollen grains and their mixtures

Authors


Correspondence to: S. M. Weidner, BAM-Federal Institute for Materials Research and Testing, Richard Willstaetter-Strasse 11, D-12489 Berlin, Germany.

E-mail: steffen.weidner@bam.de

Abstract

RATIONALE

The fast and univocal identification of different species in mixtures of pollen grains is still a challenge. Apart from microscopic evaluation and Raman spectroscopy, no other techniques are available.

METHODS

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry was applied to the analysis of extracts of single pollen grains and pollen mixtures. Pollen grains were fixed, treated and covered with matrix directly on the MALDI target.

RESULTS

Clearly resolved MALDI ion intensity images could be obtained enabling the identification of single pollen grains in a mixture.

CONCLUSIONS

Our results demonstrate the potential and the suitability of MALDI imaging mass spectrometry as an additional method for the identification of pollen mixtures. Copyright © 2013 John Wiley & Sons, Ltd.

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