Get access

An integrated exact mass spectrometric strategy for comprehensive and rapid characterization of phenolic compounds in licorice

Authors

  • Tongtong Xu,

    1. National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai, P.R. China
    Search for more papers by this author
  • Min Yang,

    Corresponding author
    • National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai, P.R. China
    Search for more papers by this author
  • Yifeng Li,

    1. National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai, P.R. China
    Search for more papers by this author
  • Xin Chen,

    1. National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai, P.R. China
    Search for more papers by this author
  • Qiurong Wang,

    1. National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai, P.R. China
    Search for more papers by this author
  • Wangping Deng,

    1. National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai, P.R. China
    Search for more papers by this author
  • Xiayan Pang,

    1. National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai, P.R. China
    Search for more papers by this author
  • Kate Yu,

    1. Waters Corporation, Milford, MA, USA
    Search for more papers by this author
  • Baohong Jiang,

    1. National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai, P.R. China
    Search for more papers by this author
  • Shuhong Guan,

    1. National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai, P.R. China
    Search for more papers by this author
  • De-an Guo

    Corresponding author
    • National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Shanghai, P.R. China
    Search for more papers by this author

Correspondence to: D.-A. Guo or M. Yang, National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Haike Road 501, Shanghai 201203, P.R. China.

E-mail: gda5958@163.com; ymn77@163.com

Abstract

RATIONALE

Licorice (Gancao) is derived from the dried roots and rhizomes of Glycyrrhiza species (Leguminosae) and appears as a component herb in about 60% of traditional Chinese medicine (TCM) prescriptions. Modern pharmacological studies have shown that flavonoids are one class of the major components responsible for the bioactivities of licorice. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/QTOF MS) has proven to be a powerful tool for rapid profiling and identification of natural products in complex herbal medicines.

METHODS

A UPLC/QTOF MS method was established for the first time for profiling and structural characterization of the phenolic compounds (most of them flavonoids) in licorice. The combined use of data-independent acquisition (MSE) and data-dependent acquisition (DDA) was illustrated.

RESULTS

Fifteen flavonoid reference compounds were used to explore the fragmentation pathways. Compound identification was based upon the exact mass, general fragmentation behaviors, retention times, UV absorption, and the related botanical biogenesis. As a result, a total of 51 compounds were characterized, three of which were reported for the first time.

CONCLUSIONS

The LC/MS analysis for each injection took less than 9 min. The developed method is fast, accurate and reliable due to its high resolution and high efficiency characteristics as a result of combining both UPLC separation and QTOF exact mass measurement. Copyright © 2013 John Wiley & Sons, Ltd.

Get access to the full text of this article

Ancillary