Quantification of intact human insulin-like growth factor-I in serum by nano-ultrahigh-performance liquid chromatography/tandem mass spectrometry
Article first published online: 22 MAY 2014
Copyright © 2014 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 28, Issue 13, pages 1426–1432, 15 July 2014
How to Cite
Lopes, F., Cowan, D. A., Thevis, M., Thomas, A. and Parkin, M. C. (2014), Quantification of intact human insulin-like growth factor-I in serum by nano-ultrahigh-performance liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom., 28: 1426–1432. doi: 10.1002/rcm.6908
- Issue published online: 22 MAY 2014
- Article first published online: 22 MAY 2014
- Manuscript Accepted: 27 MAR 2014
- Manuscript Revised: 26 MAR 2014
- Manuscript Received: 15 JAN 2014
Insulin-like growth factor-I is one of the biomarkers used to detect growth hormone administration prohibited in human sport. Current testing approaches for IGF-I rely on commercial immunoassays, which may change from time to time requiring complex revalidation. Mass spectrometry (MS)-based approaches often rely on enzymatically digesting the protein and measuring specific peptide concentrations. In order to reinforce the current available methodology for IGF-I testing, a reliable and equally sensitive MS method is required for the analysis of intact protein using small sample volumes (<25 μL).
IGF-I was extracted from human serum samples by a simple protein precipitation procedure. Separation was achieved via nano-ultrahigh-performance liquid chromatography and MS analysis was conducted by nano-electrospray ionisation triple-quadrupole mass spectrometry in the selected reaction monitoring mode using a stable-isotope-labelled internal standard.
A six-point calibration curve ranging from 50 to 1000 ng/mL of human IGF-I in rat serum was used to establish instrument response. The method provided a limit of quantification of 50 ng/mL, with intra- and inter-day precision ≤5% and intra- and inter-day accuracy ≥95%.
A quantitative method was developed for the quantification of intact IGF-I in human serum samples. The data generated provided important information for the development of a new reference method for the growth hormone biomarker test and helped create a reliable system for monitoring peptide hormones in individual athletes, a possible extension to the athlete biological passport system. Nano-electrospray has here been shown to be sufficiently robust for routine use in an analytical laboratory, allowing for the analysis of minute sample volumes. Copyright © 2014 John Wiley & Sons, Ltd.