Characterization of proanthocyanidins in grape seeds using electrospray mass spectrometry
Article first published online: 26 NOV 2002
Copyright © 2002 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 17, Issue 1, pages 9–16, 15 January 2003
How to Cite
Hayasaka, Y., Waters, E. J., Cheynier, V., Herderich, M. J. and Vidal, S. (2003), Characterization of proanthocyanidins in grape seeds using electrospray mass spectrometry. Rapid Commun. Mass Spectrom., 17: 9–16. doi: 10.1002/rcm.869
- Issue published online: 26 NOV 2002
- Article first published online: 26 NOV 2002
- Manuscript Accepted: 21 OCT 2002
- Manuscript Revised: 20 OCT 2002
- Manuscript Received: 18 SEP 2002
Two proanthocyanidin (PA) fractions, one (Sdp3) with the mean degree of polymerization (mDP) of 3 and the other (Sdp9) with mDP of 9, were obtained from a Vitis vinifera cv. Shiraz grape seed extract. The PA fractions were directly analyzed by electrospray ionization mass spectrometry (ES-MS) and negative ion mass spectra were recorded. The mass spectrum of Sdp3 exhibited only singly charged ions corresponding to the molecular mass of PA with a degree of polymerization (DP) up to 9 (nonamers). In contrast, Sdp9 yielded rather complex mass spectra featuring ions with single [M − H]−, double [M − 2H]2− and triple [M − 3H]3− charge representing the molecular masses of PAs up to a DP of 28. In addition, the degree of galloylation per procyanidin (DG) was observed to be up to 5 (pentagallates) in Sdp3 and 8 (octagallates) in the Sdp9. This is the first evidence obtained by mass spectrometry for the distribution of grape seed PAs with such a high degree of polymerization and a broad diversity of galloylation. ES-MS data together with the complementary information provided by acid hydrolysis provides a detailed picture of the composition of grape seed PAs. Copyright © 2002 John Wiley & Sons, Ltd.