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Quantification of [Dmt1]DALDA in ovine plasma by on-line liquid chromatography/quadrupole time-of-flight mass spectrometry

Authors

  • Haibao Wan,

    1. Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Health Science Center, 847 Monroe Avenue, Memphis, TN 38163, USA
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  • Dominic M. Desiderio

    Corresponding author
    1. Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Health Science Center, 847 Monroe Avenue, Memphis, TN 38163, USA
    2. Department of Neurology, University of Tennessee Health Science Center, 847 Monroe Avenue, Memphis, TN 38163, USA
    3. Department of Molecular Sciences, University of Tennessee Health Science Center, 847 Monroe Avenue, Memphis, TN 38163, USA
    • Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Health Science Center, 847 Monroe Avenue, Memphis, TN 38163, USA.
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Abstract

The synthetic peptide [Dmt1]DALDA (Dmt-D-Arg-Phe-Lys-NH2; Dmt = 2′,6′-dimethyltyrosine; ‘super-DALDA’) is a mu opioid-receptor agonist. On-line liquid chromatography/quadrupole time-of-flight mass spectrometry and the corresponding stable isotope-incorporated synthetic peptide internal standard were used to quantify [Dmt1]DALDA that had been extracted from ovine plasma samples. The [M+2H]2+ ion was used to construct the calibration curve, and the product ion was used for verification of the peptide. The detection sensitivity for the [Dmt1]DALDA [M+2H]2+ ion was 12.5 fmol and 50 fmol for the m/z 432.3 product ion. The concentration profile of [Dmt1]DALDA was determined from a set of ovine plasma samples. The molecular specificity of the peptide quantification was confirmed by tandem mass spectrometry (MS/MS). Copyright © 2003 John Wiley & Sons, Ltd.

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