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Improved detection of higher molecular weight proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on polytetrafluoroethylene surfaces

Authors

  • Catherine H. Botting

    Corresponding author
    1. Centre for Biomolecular Sciences, School of Chemistry, University of St Andrews, Fife KY16 9ST, Scotland, UK
    • School of Chemistry, University of St Andrews, Biomolecular Sciences Building, North Haugh, St Andrews, Fife KY16 9ST, Scotland, UK.
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Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of proteins was performed on a range of polytetrafluoroethylene (PTFE) surfaces. Sinapinic acid and α-cyano-4-hydroxycinnamic acid matrices were compared and the order of application varied to identify the best combination for each surface. It is demonstrated that the use of a PTFE surface improves the intensity of signals obtained for higher molecular weight proteins. Copyright © 2003 John Wiley & Sons, Ltd.

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