Atmospheric pressure chemical ionisation reversed-phase liquid chromatography/ion trap mass spectrometry of intact bacteriohopanepolyols
Article first published online: 25 FEB 2003
Copyright © 2003 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 17, Issue 7, pages 728–737, 15 April 2003
How to Cite
Talbot, H. M., Squier, A. H., Keely, B. J. and Farrimond, P. (2003), Atmospheric pressure chemical ionisation reversed-phase liquid chromatography/ion trap mass spectrometry of intact bacteriohopanepolyols. Rapid Commun. Mass Spectrom., 17: 728–737. doi: 10.1002/rcm.974
- Issue published online: 25 FEB 2003
- Article first published online: 25 FEB 2003
- Manuscript Revised: 28 JAN 2003
- Manuscript Accepted: 28 JAN 2003
- Manuscript Received: 29 NOV 2002
- The Leverhulme Trust
Atmospheric pressure chemical ionisation liquid chromatography/multi-stage ion trap mass spectrometry (APCI-LC/MSn) has been applied to the study of intact bacteriohopanepolyols. Spectral characterisation of bacteriohopanepolyols of known structure present in bacterial extracts (Zymomonas mobilis and a fermenter containing methanotrophs including Methylococcus capsulatus) has revealed greater structural detail than previous liquid chromatography/mass spectrometry (LC/MS) methods and identified characteristic fragmentations indicative of numerous biohopanoid structures. Analysis of a Recent sedimentary extract from Lake Druzhby (Antarctica) has demonstrated the power of this technique to detect biohopanoids in complex samples including at least partial characterisation of previously unknown composite structures. Copyright © 2003 John Wiley & Sons, Ltd.