These authors contributed equally to this work.
Ectopic blastema induction by nerve deviation and skin wounding: a new regeneration model in Xenopus laevis
Version of Record online: 28 MAY 2014
© 2014 The Authors. Regeneration published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 1, Issue 2, pages 26–36, April 2014
How to Cite
Mitogawa, K., Hirata, A., Moriyasu, M., Makanae, A., Miura, S., Endo, T. and Satoh, A. (2014), Ectopic blastema induction by nerve deviation and skin wounding: a new regeneration model in Xenopus laevis. Regeneration, 1: 26–36. doi: 10.1002/reg2.11
- Issue online: 20 JUL 2014
- Version of Record online: 28 MAY 2014
- Manuscript Received: 23 FEB 2014
- Scientific Research on Innovative Areas. Grant Number: 25124707
- Naito Foundation
- Accessory limb model (ALM);
- limb regeneration;
- Xenopus laevis
Recently, the accessory limb model (ALM) has become an alternative study system for limb regeneration studies in axolotls instead of using an amputated limb. ALM progresses limb regeneration study in axolotls because of its advantages. To apply and/or to compare knowledge in axolotl ALM studies to other vertebrates is a conceivable next step. First, Xenopus laevis, an anuran amphibian, was investigated. A Xenopus frog has hypomorphic regeneration ability. Its regeneration ability has been considered intermediate between that of non-regenerative higher vertebrates and regenerative urodele amphibians. Here, we successfully induced an accessory blastema in Xenopus by skin wounding and rerouting of brachial nerve bundles to the wound site, which is the regular ALM surgery. The induced Xenopus ALM blastemas have limited regenerative potential compared with axolotl ALM blastemas. Comparison of ALM blastemas from species with different regenerative potentials may facilitate the identification of the novel expression programs necessary for the formation of cartilage and other tissues during limb regeneration.