Application of fungal-based bioaugmentation was evaluated for the remediation of creosote-contaminated soil at a wood-preserving site in West Virginia. Soil at the site contained creosote-range polycyclic aromatic hydrocarbons (PAHs) at concentrations in some areas that exceed industrial risk-based levels. Two white-rot fungi (Pleurotus ostreatus and Irpex lacteus) were evaluated for remediation effectiveness in a two-month bench-scale treatability test. Both fungi produced similar results, with up to 67.3 percent degradation of total PAHs in 56 days. Pilot-scale testing was performed at the site using Pleurotus ostreatus grown on two local substrate mixtures. During the 276-day field trial, total PAHs were degraded by up to 93.2 percent, with all individual PAHs except one achieving industrial risk-based concentrations. It was recommended that fungal-based remediation be applied to all contaminated soil at the site. © 2002 Wiley Periodicals, Inc.