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Research Article
Imaging of Silkworm Meiotic Chromosome by Atomic Force Microscopy
Article first published online: 23 APR 2007
DOI: 10.1002/sca.20032
Copyright © 2007 Wiley Periodicals, Inc.
Additional Information
How to Cite
Narukawa, J., Yamamoto, K., Ohtani, T. and Sugiyama, S. (2007), Imaging of Silkworm Meiotic Chromosome by Atomic Force Microscopy. Scanning, 29: 123–127. doi: 10.1002/sca.20032
Publication History
- Issue published online: 18 JUN 2007
- Article first published online: 23 APR 2007
- Manuscript Accepted: 13 FEB 2007
- Manuscript Received: 4 DEC 2006
Funded by
- Bio-oriented Technology Research Advancement Institution (BRAIN)
- Abstract
- References
- Cited By
Keywords:
- atomic force microscopy;
- chromosome;
- pachytene;
- silkworm
Abstract
Although structural information of mitotic chromosomes has been accumulated, little information is available for meiotic chromosome structures. Here, we applied atomic force microscopy (AFM) to investigate the ultrastructures of the silkworm, Bombyx mori, meiotic pachytene chromosome in its native state with nanometer scale resolution. Two levels of DNA folding were observed on the meiotic chromosome surface, 50–70 nm granules, which were considered to be 30 nm chromatin fibers, and spherical protrusions of 400–600 nm, which were considered to be chromomeres and arranged on the surface of the chromosome parallel to the chromosome longitudinal axis. These observations suggested that AFM study is an excellent approach for obtaining information concerning the silkworm pachytene chromosome higher order structure. SCANNING 29: 000-V000, 2007. © 2007 Wiley Periodicals, Inc.