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Scanning electron microscopy of Streptomyces without use of any chemical fixatives

Authors

  • Vijay Kumar,

    1. Department of Microbiology, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and Research, Balawala, Dehradun, Uttarakhand, India
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  • Alpana Bharti,

    1. Department of Microbiology, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and Research, Balawala, Dehradun, Uttarakhand, India
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  • Omprakash Gusain,

    1. Department of Zoology and Biotechnology, H.N.B. Garhwal University, Srinagar, Uttarakhand, India
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  • Gajraj Singh Bisht

    Corresponding author
    1. Department of Microbiology, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and Research, Balawala, Dehradun, Uttarakhand, India
    • Department of Microbiology, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and Research, Balawala, Dehradun, Uttarakhand 248161, India
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Abstract

A new, short, and quick method was developed for preparation of specimen for observing Actinomycetes of genus Streptomyces by scanning electron microscopy. The cultures were directly grown on stubs and coated with a film of gold without using any fixative and dehydrating procedures. Using this simple preparation procedure, surface of intact sporing structures of Streptomyces was observed over a range of magnifications. As the preparation procedure is so simple and rapid, this procedure could be most useful for the routine examination and identification of Streptomyces. SCANNING 33:446–449, 2011. © 2011 Wiley Periodicals, Inc.

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