A new, short, and quick method was developed for preparation of specimen for observing Actinomycetes of genus Streptomyces by scanning electron microscopy. The cultures were directly grown on stubs and coated with a film of gold without using any fixative and dehydrating procedures. Using this simple preparation procedure, surface of intact sporing structures of Streptomyces was observed over a range of magnifications. As the preparation procedure is so simple and rapid, this procedure could be most useful for the routine examination and identification of Streptomyces. SCANNING 33:446–449, 2011. © 2011 Wiley Periodicals, Inc.