H. Jin and H. Zhao contributed equally to this work.
Apoptosis Induction of K562 Cells by Lymphocytes: An AFM Study
Article first published online: 29 MAY 2012
© Wiley Periodicals, Inc.
Volume 35, Issue 1, pages 7–11, January-February 2013
How to Cite
Jin, H., Zhao, H., Liu, L., Jiang, J., Wang, X., Ma, S. and Cai, J. (2013), Apoptosis Induction of K562 Cells by Lymphocytes: An AFM Study. Scanning, 35: 7–11. doi: 10.1002/sca.21028
Contract grant sponsor: 973 Program Projects; Contract grant number: 2010CB833603; Contract grant sponsor: Overseas, Hong Kong & Macao Scholars Collaborated Researching Fund; Contract grant number: 31129002; Contract grant sponsor: National Natural Science Foundation of China; Contract grant number: 81071491; Contract grant sponsor: Fundamental Research Funds for the Central Universities.
- Issue published online: 15 FEB 2013
- Article first published online: 29 MAY 2012
- Manuscript Accepted: 12 APR 2012
- Manuscript Revised: 10 APR 2012
- Manuscript Received: 14 MAR 2012
- 973 Program Projects. Grant Number: 2010CB833603
- Overseas, Hong Kong & Macao Scholars Collaborated Researching Fund. Grant Number: 31129002
- National Natural Science Foundation of China. Grant Number: 81071491
- Fundamental Research Funds for the Central Universities
- K562 cells;
- atomic force microscope
Antitumor immunotherapies, as a prospective approach for local cancer treatment, are attracting increasing interests. To detect the reacting course of immune and tumor cells, we have observed the process of K562 cells (a human erythroleukemic cell line) coculturing with peripheral lymphocytes, and the morphological and ultrastructural alterations of K562 cells and lymphocytes were investigated as well using atomic force microscopy (AFM). AFM morphological imaging revealed that after coculture the apoptosis-like structures such as blebbing, pores, and apoptotic bodies were observed on the K562 cells. Also, the cell-surface roughness decreased significantly, which implied the changes in chemical composition of cell membranes. Moreover, the lymphocytes were damaged to some extent induced by the coculture. The data demonstrated that K562 cells could be attacked and induced apoptosis by lymphocytes, and they would make damages to lymphocytes to escape the surveillance of immune system. SCANNING 35:7-11, 2013. © 2012 Wiley Periodicals, Inc.