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Second Harmonic Generation in 3-D Uniform Arrangement of Type I Collagen on Nonlinear Optics Microscopy

Authors

  • Z. F. Zhuang,

    Corresponding author
    • MOE Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China
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  • M. F. Zhu,

  • Z.Y. Guo,

  • S. H. Liu


  • Contract grant sponsor: Foundation for the Construction of Strong Province in Traditional Chinese Medicine Administration; Contract grant sponsor: Department of Traditional Chinese Medicine of Guangdong Province; Contract grant number: 20112179; Contract grant sponsor: National Natural Science Foundation of China; Contract grant number: 60778047; Contract grant sponsor: Natural Science Foundation of Guangdong Province; Contract grant number: 9251063101000009; Contract grant sponsor: Specialized Research Fund for the Doctoral Program of Higher Education of China; Contract grant number: 200805740003

Address for reprints: Z. F. Zhuang, MOE Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China E-mail: zhuangzhf@yahoo.com.cn

Summary

Second harmonic microscopic imaging and spectroscopy technology has become a powerful tool for biomedical studies, especially in fibrosis-related diseases research. And type I collagen is the major risk factors for fibrotic diseases. In this study, model for three-dimensional (3-D) uniform arrangement type I collagen is set up for researching the second harmonic generation (SHG) on nonlinear optics microscopy. Based on this model, we discuss the influence of different length and size collagen in 3-D arrangement type I collagen. Results can guide us to neatly judge the size, length, and molecules density effect on SHG. For practical application, this theoretical approach can lead us to analyze different severity of collagen diseases. SCANNING 35:12-16, 2013. © 2012 Wiley Periodicals, Inc.

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