Visualization of Epidermal and Dermal Alteration in Papulonodular Mucinosis by Multiphoton Microscopy

Authors

  • Xiaoqin Zhu,

    1. Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China
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  • Lihang Lin,

    1. Department of Dermatology, Affiliated Union Hospital Fujian Medical University, Fuzhou, People's Republic of China
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  • Haibo Yu,

    1. Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China
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  • Shuangmu Zhuo,

    1. Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China
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  • Jianxin Chen,

    Corresponding author
    • Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China
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  • Jingjing Liu,

    1. Department of Dermatology, Affiliated Union Hospital Fujian Medical University, Fuzhou, People's Republic of China
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  • Yuying Wang

    1. Department of Dermatology, Affiliated Union Hospital Fujian Medical University, Fuzhou, People's Republic of China
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  • Contract grant sponsor: National Natural Science Foundation of China; Contract grant numbers: 81101209, 60908043, 30970783; Contract grant sponsor: Natural Science Foundation of Fujian Province; Contract grant number: 2010J01321; Contract grant sponsor: Natural Science Funds for Distinguished Young Scholar in Fujian Province; Contract grant number: 2009J06031; Contract grant sponsor: Program for Changjiang Scholars and Innovative Research Team in University; Contract grant number: IRT1115.

  • XZ and LL contributed equally to this work.

Address for reprints: Jianxin Chen, Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China E-mail: chenjianxin@fjnu.edu.cn

Summary

Papulonodular mucinosis (PM) is a cutaneous clue to the presence and activity of silent lupus erythematosus (LE), but the exact pathogenesis is still under secret. Moreover, the currently available treatments for PM are not satisfactory. To demonstrate the possibility of multiphoton microscopy (MPM) to trace the pathological state of PM and evaluate the treatment efficacy, epidermal and dermal alteration in skin lesion with PM before and after treatment was examined using MPM. Microstructure of epidermis as well as content and distribution of collagen and elastin in dermis were quantified to characterize the pathological states of PM. The results showed significant morphological difference between skin lesion before and after treatment, indicating the possibility of MPM to assess the therapeutic efficacy. With the advancement on MPM miniaturization and enhancement of contrast and depth of imaging, the MPM technique can be applied in in vivo tracking PM formation and progression, and leading the better understanding the PM pathogenesis and mechanism of response to any treatment, helping to establish novel effective therapies for PM. SCANNING 35:22-27, 2013. © 2012 Wiley Periodicals, Inc.

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