Roughness statistical influence on cell adhesion using profilometry and multiscale analysis

Authors

  • Sylvain Giljean,

    1. Laboratoire Physique et Mécanique Textile (LPMT), EA 4365, Université de Haute-Alsace, Mulhouse, France
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  • Maxence Bigerelle,

    1. LAMIH /TEMPO, Université de Valenciennes et du Hainaut Cambrésis, Le Mont Houy, Valenciennes, France
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  • Karine Anselme

    Corresponding author
    1. Institut de Science des Matériaux de Mulhouse (IS2M), CNRS LRC7228, Université de Haute-Alsace, Mulhouse, France
    • Address for reprints: Karine Anselme, Institut de Science des Matériaux de Mulhouse (IS2M), CNRS LRC7228, Université de Haute-Alsace, 15 rue Jean Starcky, BP 2488, 68057 Mulhouse Cedex, France.

      E-mail: karine.anselme@uha.fr

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  • Contract grant sponsor: Centre Technique des Industries Mecaniques.

Summary

In this study, two series of 11 samples of TiAl6V4 titanium alloy and 316L stainless steel have been polished in an isotropic manner at different levels in order to quantify the influence of biomaterial roughness on cell behavior. Topography was measured by a tactile profilometer and a multiscale analysis has been carried out. Human osteoblasts have been cultured on those samples. It appears that roughness has no reproducible effect on the cell behavior except an influence on cell orientation on the wider grooves. As a conclusion, biomaterial surface damage, in the roughness range between Ra = 0.01 and 0.1 μm, has no influence on cell-adhesion mechanisms when roughness is isotropic and groove width is inferior to a critical value. SCANNING 36:2–10, 2014. © 2012 Wiley Periodicals, Inc.

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