We briefly describe the advantages and limitations of label-free multiphoton microscopy and probe-labeled two-photon microscopy for the endomicroscopic diagnosis. The two methods are complementary and more information can be collected from tissues by combining the two methods. Therefore, parallel efforts should be directed to the development of both label-free MPM and probe-labeled TPM as the diagnostic tool. SCANNING 36:462–464, 2014. © 2013 Wiley Periodicals, Inc.