Information of multi-cells is big data because of the enormous quantities of various cells as well as their parameters and status. To securely and efficiently integrate all the cells' information and trace multi-cells are challenging due to varying number of the multi-cells, as well as the complicacy of the multi-cells' movement. In this paper, an automatic big data integration algorithm based on the optical transfer function is proposed. The experimental results show that the algorithm can securely and efficiently integrate all the cell information and simultaneously track a large quantity of cells. Copyright © 2013 John Wiley & Sons, Ltd.