SIMS proceedings paper
Novel method to prepare biological samples using powerful magnets on TOF-SIMS analysis
Article first published online: 14 AUG 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Surface and Interface Analysis
Special Issue: Proceedings of the Eighteenth International Conference on Secondary Ion Mass Spectrometry, SIMS XVIII, Riva Del Garda, Trento, Italy, September 18 - 23, 2011
Volume 45, Issue 1, pages 248–250, January 2013
How to Cite
Chang, C.-C., Chen, C.-N., Lin, W.-J., Lo, T.-Y., Lei, S.-L. and Mai, F.-D. (2013), Novel method to prepare biological samples using powerful magnets on TOF-SIMS analysis. Surf. Interface Anal., 45: 248–250. doi: 10.1002/sia.5148
- Issue published online: 18 DEC 2012
- Article first published online: 14 AUG 2012
- Manuscript Accepted: 3 JUL 2012
- Manuscript Revised: 25 JUN 2012
- Manuscript Received: 8 OCT 2011
- powerful magnets;
Imaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has been shown to be a powerful analytical tool for mapping the distribution of biologically relevant small molecules (<1000 Da) on a surface. To improve the success rate of sample preparation, a device was designed to cells freeze-fracture by using powerful magnets. Quantum dots (QDs) were used to observe the changes in the cell after freeze-fracture. Then, TOF-SIMS was employed to analyze the ionic and molecular distribution in the cells associated with the disease. Copyright © 2012 John Wiley & Sons, Ltd.