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Novel method to prepare biological samples using powerful magnets on TOF-SIMS analysis

Authors

  • Chun-Chao Chang,

    1. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan, ROC
    2. Department of Internal Medicine, School of Medicine, Taipei Medical University, Taipei, Taiwan, ROC
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  • Chun-Nan Chen,

    1. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Wan Fang Hospital, Taipei, Taiwan, ROC
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  • Wei-Jhih Lin,

    1. Graduate Institute of Medical Science, Taipei Medical University, Taipei, Taiwan, ROC
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  • Tsui-Yun Lo,

    1. Department of Biochemistry, School of Medicine, Taipei Medical University, Taipei, Taiwan, ROC
    2. Biomedical Mass Imaging Research Center, Taipei Medical University, Taipei, Taiwan, ROC
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  • Shiou-Ling Lei,

    1. Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan, ROC
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  • Fu-Der Mai

    Corresponding author
    1. Graduate Institute of Medical Science, Taipei Medical University, Taipei, Taiwan, ROC
    2. Biomedical Mass Imaging Research Center, Taipei Medical University, Taipei, Taiwan, ROC
    • Department of Biochemistry, School of Medicine, Taipei Medical University, Taipei, Taiwan, ROC
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Fu-Der Mai, Department of Biochemistry, School of Medicine, Taipei Medical University, Taipei 11031, Taiwan, ROC.

E-mail: fordmai@tmu.edu.tw

Abstract

Imaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has been shown to be a powerful analytical tool for mapping the distribution of biologically relevant small molecules (<1000 Da) on a surface. To improve the success rate of sample preparation, a device was designed to cells freeze-fracture by using powerful magnets. Quantum dots (QDs) were used to observe the changes in the cell after freeze-fracture. Then, TOF-SIMS was employed to analyze the ionic and molecular distribution in the cells associated with the disease. Copyright © 2012 John Wiley & Sons, Ltd.

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