SIMS proceedings paper
ToF-SIMS imaging of lipids and lipid related compounds in Drosophila brain
Article first published online: 13 JUN 2014
Copyright © 2014 John Wiley & Sons, Ltd.
Surface and Interface Analysis
How to Cite
Phan, N. T. N., Fletcher, J. S., Sjövall, P. and Ewing, A. G. (2014), ToF-SIMS imaging of lipids and lipid related compounds in Drosophila brain. Surf. Interface Anal.. doi: 10.1002/sia.5547
- Article first published online: 13 JUN 2014
- Manuscript Accepted: 11 APR 2014
- Manuscript Revised: 2 APR 2014
- Manuscript Received: 26 NOV 2013
- imaging mass spectrometry;
Drosophila melanogaster (fruit fly) has a relatively simple nervous system but possesses high order brain functions similar to humans. Therefore, it has been used as a common model system in biological studies, particularly drug addiction. Here, the spatial distribution of biomolecules in the brain of the fly was studied using time-of-flight SIMS. Fly brains were analyzed frozen to prevent molecular redistribution prior to analysis. Different molecules were found to distribute differently in the tissue, particularly the eye pigments, diacylglycerides, and phospholipids, and this is expected to be driven by their biological functions in the brain. Correlations in the localization of these molecules were also observed using principal components analysis of image data, and this was used to identify peaks for further analysis. Furthermore, consecutive analyses following 10 keV Ar2500+ sputtering showed that different biomolecules respond differently to Ar2500+ sputtering. Significant changes in signal intensities between consecutive analyses were observed for high mass molecules including lipids. Copyright © 2014 John Wiley & Sons, Ltd.