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Simple Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica Materials Hosting Crosslinked Enzyme Aggregates

  1. Jinwoo Lee Dr.2,
  2. Jungbae Kim Dr.1,
  3. Jaeyun Kim2,
  4. Hongfei Jia3,
  5. Moon Il Kim4,
  6. Ja Hun Kwak Dr.1,
  7. Sunmi Jin2,
  8. Alice Dohnalkova1,
  9. Hyun Gyu Park Prof.4,
  10. Ho Nam Chang Prof.4,
  11. Ping Wang Prof. Dr.3,
  12. Jay W. Grate Dr.1,
  13. Taeghwan Hyeon Prof. Dr.2

Article first published online: 11 MAY 2005

DOI: 10.1002/smll.200500035

Issue

Small

Small

Volume 1, Issue 7, pages 744–753, July 2005

Additional Information

How to Cite

Lee, J., Kim, J., Kim, J., Jia, H., Kim, M., Kwak, J., Jin, S., Dohnalkova, A., Park, H., Chang, H., Wang, P., Grate, Jay W. and Hyeon, T. (2005), Simple Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica Materials Hosting Crosslinked Enzyme Aggregates. Small, 1: 744–753. doi: 10.1002/smll.200500035

Author Information

  1. 1

    Pacific Northwest National Laboratory, Richland, WA 99352, USA, Fax: (+1) 509-375-5106

  2. 2

    National Creative Research Initiative Center for Oxide, Nanocrystalline Materials and School of Chemical Engineering, Seoul National University, Seoul 151-744, Korea, Fax: (+82) 2-888-1604

  3. 3

    Department of Chemical Engineering, University of Akron, Akron, Ohio 44325, USA

  4. 4

    Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea

Publication History

  1. Issue published online: 8 JUN 2005
  2. Article first published online: 11 MAY 2005
  3. Manuscript Received: 27 JAN 2005

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Keywords:

  • crosslinking;
  • enzymes;
  • mesoporous materials;
  • silica;
  • template synthesis

Abstract

Hierarchically ordered mesocellular mesoporous silica materials (HMMS) were synthesized using a single structure-directing agent. The mesocellular pores are synthesized without adding any pore expander; the pore walls are composed of SBA-15 type mesopores. Small-angle X-ray scattering revealed the presence of uniform pore structures with two different sizes. Using HMMS as a nanoscopic template, hierarchically ordered mesocellular mesoporous carbon (HMMC) and polymer (HMMP) materials were synthesized. HMMS was used as a host for enzyme immobilization. To improve the retention of enzymes in HMMS, we adsorbed enzymes, and then employed crosslinking using glutaraldehyde (GA). The resulting crosslinked enzyme aggregates (CLEAs) show an impressive stability with extremely high enzyme loadings. For example, 0.5 g α-chymotrypsin (CT) could be loaded in 1 g of silica with no activity decrease observed with rigorous shaking over one month. In contrast, adsorbed CT without GA treatment resulted in a lower loading, which further decreased due to continuous leaching of adsorbed CT under shaking. The activity of crosslinked CT aggregates in HMMS was ≈10 times higher than that of the adsorbed CT, which represents a 74-fold increase in activity per unit weight of HMMS due to higher CT loading.

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